Single-cell ATP detection and content analyses in electrostimulus-induced apoptosis using functionalized glass nanopipettes.
Plasmonic SERS Au Nanosunflowers for Sensitive and Label-Free Diagnosis of DNA Base Damage in Stimulus-Induced Cell Apoptosis.
Time optimization for cells apoptosis by ES, the SERS spectrum of MBA and assignment, the contents of MBA and aptamer to Cyt c absorbed on AuNPs estimated, the UV–vis spectra of smart plasmonic nanorobot before and after capturing Cyt c and capture rate investigated, the SEM images and SERS spectra of MBA-Au before and after ES, the selectivity of nanorobot, the stability of Raman instrument, the mitochondrial membrane potential images of MCF-7 cells in different time under ES, the flow cytometry analysis of MCF-7 cells before and after ES treatment, the fluorescence imaging of ROS within MCF-7 cells treated with different voltages, the nanorobot uptaken by MCF-7 cells with different time and the contents distributed into cytoplasm and lysosomes, Bio-TEM images of MCF-7 cells incubated with nanorobots, the SERS monitoring of Cyt c and pH changes in living cells with different time under the ES (0.9 V), and the repeatability of nanorobots in response to different cells.
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The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.analchem.8b04027.